1) Select a clean room. Sanitation is not a major concern at this stage, but do make sure that there aren’t any fans running, windows open, or pets roaming around. You will do an “open-air” spawn where the colonized grain will be mixed with the substrate. The area should still be clean (i.e., you have a clean surface available to work on, there isn’t any mold present in the room, and so on.)

2) Give the fruiting chamber bin—the “monotub”—a liberal wipe down with a paper towel moistened with alcohol. 70% isopropyl rubbing alcohol is great. Allow a few minutes to dry.

3) Place a square of micropore tape over the inside and outside of each pre-drilled hole of the fruiting chamber. Alternatively, you can use polyfill stuffing, available at any craft store or as included in our Complete All-In-One Mushroom Grow Kit. Stuff the holes of the fruiting chamber with the polyfill.

4) Lining the Fruiting Chamber:

Method 1- Line the interior: Fit the bin liner into the interior of the fruiting chamber. Trim the liner so it only rises up about 4 inches on the sides of the fruiting chamber. Masking tape can be helpful to temporarily hold the liner in place.

Method 2- Line the exterior: Fit the bin liner onto the exterior of the fruiting chamber. Trim the liner so it only rises up about 4 inches on the sides of the fruiting chamber. Secure the liner to the outside of the bin with masking or duct tape.

5) Using your hands, break up a block of colonized grain spawn in its bag. Ensure that the bag is white. Once it is thoroughly broken up, use scissors to cut open the bag and dump it into the fruiting chamber.

6) Cut open your Super Shroom substrate and dump it into the fruiting chamber as well.

7) Using your clean hands, preferably while wearing latex gloves, thoroughly mix the substrate and grain spawn. Once mixed, spread evenly throughout the fruiting chamber. The surface should be somewhat level.

8) Fasten the lid to the fruiting chamber.

9) Place the fruiting chamber in a room that receives natural lighting and is between 70-75 degrees.


After your fruiting chamber is set up, it will take 2-3 weeks to fully colonize.

An important step in your mushroom growing equipment procedure is to monitor your tub for dryness. This is critical! If you see lighter patches of substrate, lightly mist with a spray bottle of clean tap water until darkened. After the substrate is fully colonized, it will be important to continue to monitor for surface moisture. There should always be very small beads of water on the surface of your substrate. If you don’t see these, mist lightly once a day until small beads of water are visible. After you mist, fan the substrate for 20-30 seconds. This is to initiate evaporation, a key pinning trigger.


After you set up your fruiting chamber. It should not need any attention for at least two weeks. After this point, you should set up your humidity control kit. Set up the humidifier with the hose attachments and fit the end of the hose into the highest hole on the side of the fruiting chamber that has three holes. Remember to fill the humidifier with water. Program the timing switch to turn on for one minute each day. Plug the humidifier into the timing switch. That’s it! Set it and forget it!


If your room is colder than 70 degrees, you will want to employ the heating mat. Simply set up the mat on a flat surface and turn it on. Place a towel or piece of cardboard on top of the mat, then place the fruiting chamber on top. Monitor the temperature in the fruiting chamber (it should not rise above 78 degrees). If it is too hot, add more layers of cardboard until the appropriate temperature zone is reached (between 70-75 degrees).


Your fruiting chamber should begin to produce fruits 2-3 weeks after becoming fully colonized. Mushrooms will begin to pin-up and then grow quickly. You should harvest your mushrooms when the cap flutes out and the veil breaks, exposing the gills of the mushrooms. Harvest your mushrooms by twisting and pulling them from the substrate.

A flush of mushrooms includes all of the mushrooms that are produced in the same general wave. Once your first flush is harvested, the substrate can be “reset” for additional flushes. Gently pour tap water on top of the substrate until a small puddles form. Now pour water down the sides of the substrate to a depth of about ½ inch. After 24 hours, dump off the excess water and resume regular misting and fanning.


Use the included dehydration kit to effectively dry out your mushrooms. Remove everything from the plastic container, add the desiccant beads until they form a layer about a ¼ inch thick. Lay the screen on top of the beads, then add your mushrooms on top of the screen. Drying may take a few days. Once dry to the touch, store your mushrooms in the included mason jar. Note: Storing mushrooms in the refrigerator will extend their life.


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